Potato Arabinogalactan Acts as Prebiotics by A Simple Extraction

The effect of the extracting procedures for potato pulp arabinogalactan were investigated on growth gut-microflora by in vitro experiments. Crude saccharides (CS) prepared 50 g dried suspended 1 L pure water. suspensions autoclaved at 1211C 2 h, and filtered a filter paper. filtrate concentrated around 1/4 rotary evaporator, aggregated 4× volume ethanol. Dialyzed (DS) to dialyzed CS dialysis membrane (cut off MW < 10,000). Enzyme-treated (ES) digest crude amylase, Gruku-Gin (Amano Enzyme) dialyzed. All lyophilized or heat-dried store. According size-exclusion chromatography, molecular distribution between 400 200,000. A peak oligosaccharides was observed 800. disappeared charts DS ES. Glucan occupied 50% total amount glycan DS, decreased approx. 5% When thirteen strains gut microbes cultivated GAM broth including instead glucose, CP EP selectively stimulated several non-pathogenic Bifidobacterium Clostridium , but not pathogenic Enterobacteriaceae .